Comet Assay measurements: a perspective
نویسندگان
چکیده
منابع مشابه
The comet assay: a heavenly method!
The contributions to this special issue of Mutagenesis have been selected to cover the main research areas served by the comet assay, namely genotoxicology, environmental toxicology, human biomonitoring and fundamental investigations into mechanisms of DNA damage and repair. Innovative methods are described, technical issues are explored, and guidelines are given for venturing into relatively n...
متن کاملComet assay as a sensitive technique in occupational health studies; A literature review
Background: Occupational health is a sensitive field in many countries where occupational exposure needs attention due to the lack of awareness. Several businesses, including leather, textile, iron, steel, and dyeing industries use a variety of mutagenic and genotoxic chemicals. Exposure to these chemicals may pose serious health risks, including genetic damage to workers. Comet assay is a tech...
متن کاملIs Enceladus a Comet? a Cometary Perspective
Introduction: The discovery of icy plumes emanating from Saturn's moon Enceladus by the Cassini spacecraft has raised questions about the cometary nature of this small satellite. The release of gas and dust from cometary nuclei is restricted to " jets " or plumes also and this activity has been observed in comets at distances much further than Saturn's orbit, including comet-like activity and a...
متن کاملComet assay in myelodysplastic syndromes
The comet assay (single-cell gel electrophoresis) has been established as a simple, rapid, flexible and sensitive method of detecting DNA damage in single cells(1,2). Cells embedded in agarose on a microscope slide are lysed with detergent. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the intensity of the comet tail relative to the hea...
متن کاملRecommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop.
The in vivo alkaline single cell gel electrophoresis assay, hereafter the Comet assay, can be used to investigate the genotoxicity of industrial chemicals, biocides, agrochemicals and pharmaceuticals. The major advantages of this assay include the relative ease of application to any tissue of interest, the detection of multiple classes of DNA damage and the generation of data at the level of th...
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ژورنال
عنوان ژورنال: Cell Biology and Toxicology
سال: 2007
ISSN: 0742-2091,1573-6822
DOI: 10.1007/s10565-007-9043-9